How To Make A Magic Mushroom Spore Syringe - Zamnesia USA (2024)

How To Make A Magic Mushroom Spore Syringe - Zamnesia USA (1)

How To Make A Magic Mushroom Spore Syringe - Zamnesia USA (2)

Luke Sumpter

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There are a couple of ways to inoculate a substrate to grow magic mushrooms. From ghostly liquid cultures to Petri dishes, it can be hard to decide which method is best when starting out. Using a spore syringe is one of the simplest and cleanest options—a perfect solution for the novice grower!

Growing your own magic mushrooms is a deeply rewarding process. It is also a very involved process with numerous steps, each of which must be done carefully and with precision. The very first of these is inoculation of the substrate, followed by germination of your mushroom spores. For first-time growers, the careful, hands-on methods required can be equal parts daunting and exciting. But, we’re here to help; let’s explore how to make your very first magic mushroom spore syringe.

What Is a Spore Syringe?

A spore syringe is an essential part of growing your own magic mushrooms—it is used to inoculate your chosen substrate. The minuscule spores floating about in the spore solution hold the genetic code to the (hopefully) ounces and ounces of magic mushrooms you can expect to grow. Mixing your spores into sterile water inside a syringe is one of the most effective, and easiest, germination techniques when growing mushrooms.

Why Use a Spore Syringe?

Using a spore syringe is about the easiest way to maintain cleanliness in your mushroom grow, especially if used alongside a flow hood. There are other methods of inoculation, such as liquid cultures, which will be covered later. Generally though, a spore syringe allows spores to be added directly to the substrate without a huge risk of contamination (when done properly).

If you’re a first-time grower, it’s hard to beat this method. Simple and reliable, it’s an easy process for the novice. Indeed, many seasoned cultivators continue to opt for this method.

How To Make a Spore Syringe

How To Make A Magic Mushroom Spore Syringe - Zamnesia USA (5)

To make a spore syringe, you’ll first need to source some magic mushroom spores. These can be found online in the form of spore prints, or you can buy ready-made spore syringes.

To avoid the risk of contamination, prepare a clean environment with as little airflow as possible. It is recommended to do this in a still air box or with a laminar flow hood. Failing this, use a small room with surfaces which can be bleached first. Consider wearing gloves and a mask, too.

Although, be aware that this stage does not present the greatest risk of contamination, so there’s no need to go overboard. However, any extra care you take will pay off, as growing mushrooms is a long and fairly involved process, and there’s little worse than finding a cake covered in mould two months down the line!

All of the equipment listed below should be easily accessible, either online or in a shop. Moreover, it can be a fairly cheap setup, with the reward far outweighing the cost. And, besides the spores, none of it is suspect or controlled. Fortunately, in most countries, even spore prints from psilocybin mushrooms are not illegal.

Equipment for a Spore Syringe

How To Make A Magic Mushroom Spore Syringe - Zamnesia USA (6)

  • Spore print
  • Sterile syringe (10–20ml should be adequate)
  • Sterile water
  • Shot glass or small dish
  • Pressure cooker
  • Scalpel (optional: any hard, clean edge will suffice)
  • Tweezers
  • Gas hob or burner
  • Ziplock bag

Spore Syringe Step-By-Step Directions

How To Make A Magic Mushroom Spore Syringe - Zamnesia USA (7)

How To Make A Magic Mushroom Spore Syringe - Zamnesia USA (8)

1. Start out by sterilising your water and shot glass/small dish. Place in your pressure cooker and heat until it reaches 15psi. Once you've reached pressure, lower the temperature and continue to cook for at least 30 minutes. This amount of time will ensure adequate sterilisation (if reusing syringes, they can be added in this stage too).

How To Make A Magic Mushroom Spore Syringe - Zamnesia USA (9)

2. Turn off the heat completely after 30 minutes and allow the water to cool to room temperature. This stage is very important; if you proceed while the water is still hot, you run a huge risk of killing the spores. Your equipment can be as sterile as you'd like, but if you cook the spores it’s all pointless.

It should take a few hours to completely cool, but it’s safest to let it sit over night. Make sure you leave it sealed! If you open it before you are about to use the equipment, it will become contaminated again.

How To Make A Magic Mushroom Spore Syringe - Zamnesia USA (10)

3. Now you are ready to make the spore syringes. Sterilise your tweezers and scalpel using a flame.

How To Make A Magic Mushroom Spore Syringe - Zamnesia USA (11)

How To Make A Magic Mushroom Spore Syringe - Zamnesia USA (12)

4. Remove the shot glass/small dish from the pressure cooker.

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5. With your tweezers, take the spore print from its storage and hold it over the opening of the shot glass/small dish.

How To Make A Magic Mushroom Spore Syringe - Zamnesia USA (14)

6. With scalpel in hand, carefully scrape some of the spores into the water.

How To Make A Magic Mushroom Spore Syringe - Zamnesia USA (15)

How To Make A Magic Mushroom Spore Syringe - Zamnesia USA (16)

7. You should fill your syringes instantly—place the tip of your first syringe into the water and fill it up. It is a good idea to empty the syringe back into the shot glass and repeat this process a couple of times. This will ensure there is a good spread of spores throughout the water—do this every time you add more spores.

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8. Now that you have a filled syringe, you need to leave it at room temperature for 2–3 days so the spores can fully hydrate.

How To Make A Magic Mushroom Spore Syringe - Zamnesia USA (18)

9. Once hydrated, you can store your spore syringes in an airtight ziplock bag in the fridge. They will remain viable for up to 12 months.

How To Use a Spore Syringe

How To Make A Magic Mushroom Spore Syringe - Zamnesia USA (19)

Using a spore syringe is fairly simple, so long as you exercise restraint. There are literally millions of spores in each syringe, so you shouldn’t need more than 3 millilitres per square litre of substrate. That’s about a drop or two.

If you’re inoculating a substrate in a jar, it’s most likely the jar will have holes punched through the lid, sealed with tape or a plug of some sort. Whatever the case, the process is the same. Pierce the microporous tape that should be covering these holes (or remove the plug) and squirt a little of the spore solution onto the substrate. How much depends on the size of the jars.

Once this is complete, place new microporous tape over the pierced strips and place the jars in a suitable warm and dark location. Hopefully, within a week you should begin to see mycelium colonising the places where you dropped the spore solution. It should look like little patches of cotton wool.

Over the following weeks, these patches should grow to envelop the entire jar. Do not do anything with it until it is fully colonised! Likewise, keep an eye on what else is growing in there to see if it's been contaminated.

How To Store a Spore Syringe

How To Make A Magic Mushroom Spore Syringe - Zamnesia USA (20)

Stored properly, spore syringes can last up to 12 months by some accounts. Remember that the spores are living things, therefore inadequate storage will kill them.

Do Spore Syringes Need To Be Refrigerated?

Yes. Spore syringes should always be refrigerated at between 2°C and 8°C. They should also be stored in darkness, although this likely shouldn’t be a problem as they will be kept in a fridge.

Can Spore Syringes Be Frozen

No. Spores are cells that contain water. Therefore, freezing will destroy them.

Do Spore Syringes Go Bad?

Yes. If stored poorly, they can die. Likewise, they will naturally die in time too, although they are surprisingly resilient little things when stored well.

Can You Reuse a Spore Syringe?

Absolutely. So long as you sterilise the syringe properly, and it is not damaged, there is no reason one cannot be used many times. To sterilise a syringe, either fill it with sterilised boiling water or cook it in a pressure cooker. To sterilise the needle, use a flame.

Spore Syringes Versus Liquid Cultures

How To Make A Magic Mushroom Spore Syringe - Zamnesia USA (21)

Liquid cultures are another popular way of inoculating substrates with mushroom spores. Essentially, the germination happens within a liquid substrate, and you’ll find the mycelium ethereally suspended. Liquid cultures happen in a nutrient-rich “broth” (sterile water enriched with sugars).

With liquid cultures, most believe it is important to isolate a single strain, otherwise you will lack consistency and control down the line, and the strains will compete with one another. Spore syringes, on the other hand, can be single or multispore.

Hawaii Liquid Culture Syringe (Acid Shroomz)



Spore Syringe Versus Spore Prints in Agar

How To Make A Magic Mushroom Spore Syringe - Zamnesia USA (23)

Another germination method is within an agar solution in a Petri dish. Agar is a sugar-rich compound in which spores can germinate and mycelium can grow. It too is a simple method, as you can drop spores from a spore print directly onto it.

However, if it is not done in a sealed air box or under a flow hood, there is a very high risk of contamination, as the Petri dish will be uncovered, allowing many other microbes onto its surface. The environment in which mycelium likes to grow is the same that many other organisms love!

Related articleHow To Take Magic Mushroom Spore Prints

Which method you choose is down to preference and ease. Spore syringes are the simplest way to go, and are advisable for first-time growers. Due to the simplicity and speed of the process, it’s just an easy method. However, the germination process within the substrate is slower than using a liquid culture.

Liquid cultures, while more difficult to make, do tend to yield better results. Basically, because the germination has happened and you already have mycelium, it colonises the substrate far faster and more aggressively than the spore syringe method. What this means is that you mitigate the risk of contamination, as the mycelium has a greater chance of being dominant. As spore syringes are slower to colonise, this allows a bigger window for competitive growth.

Where Can You Find Spore Syringes?

Both spore syringes and liquid cultures are available online. Although, it’s important to check how reputable the sources are and the laws in your country. In some nations, the mycelium of magic mushrooms is illegal, even where the spores aren’t.

But, if you’re interested in growing, it’s worth giving all methods a go yourself. When you harvest your first massive flushes some months later, you’ll look back fondly at the very first steps. Plus, you’ll already know how to immediately make new ones from your very own spores!

How To Make A Magic Mushroom Spore Syringe - Zamnesia USA (2024)


How do you make a mushroom spore syringe? ›

The process of making a spore syringe
  1. Gather the right materials. ...
  2. Sterilize your water by boiling it. ...
  3. Grab your spore print. ...
  4. Scrape spores from the print into the sterile syringe carefully.
  5. Add the cooled, boiled water to the syringe. ...
  6. Shake the syringe gently.
Feb 29, 2024

How to make mushroom spores at home? ›

Collecting Spores:
  1. Choose a mature mushroom of a known edible variety.
  2. Carefully remove the stem and any skirt protecting the gills.
  3. Place the mushroom cap with the gills facing down on a piece of paper.
  4. Cover the cap with a glass and leave it undisturbed for 24 hours.
Aug 11, 2022

How much spore solution to use? ›

We recommend to use about 10 - 20 ml – that is 1 - 2 syringes - for small bags (2,5 L content). For larger bags (4,5 L content) we recommend to use 20 - 30 ml spore solution – that is 2 - 3 spore syringes. ATTENTION: Should the needle get in contact with any unsterile material, you will have to heat-sterilize it.

How do you get liquid culture into a syringe? ›

Using your liquid culture syringe
  1. Open the box and remove the liquid culture syringe and the needle from the box. ...
  2. Shake the syringe or gently flick it so that the mycelium mixes with the liquid in the syringe. ...
  3. Remove the protection cap from the edge of the syringe. ...
  4. Open the package containing the injection needle.

How do you make a spore solution? ›

How to prepare spore suspension?
  1. Pipet 6-8ml dH2O to a well-sporulating plate (2-3ml for slant cultures).
  2. Scrape of growth with a sterile loop to release spores.
  3. Collect solution off the top of the plate using a pipet and transfer all liquid into a sterile 15 conical tube.
Jul 11, 2017

How long does it take for a spore syringe to colonize? ›

Colonization occurs after inoculation. This is when the mycelium will start to take over the grain that was inoculated with spores. This period usually lasts between 3 to 6 weeks depending on strain and environmental conditions. Ideal conditions for this phase are in a dark place and temperature between 72 F – 80 F.

How do you germinate mushroom spores? ›

Choose a suitable substrate for your mushroom species. Brown rice flour and vermiculite are popular for p. cubensis. Mix water with the brown rice flour to create a moist substrate that will support spore germination.

What do mushroom spores need to germinate? ›

They need, in addition to water, a physical or chemical activator. 1 This is why all damp surfaces are not covered with fungi growth after a rain or dew. If a spore has been activated but dries up, the spore will remain activated and as soon as conducive environmental conditions arise the spore germinates.

How do you extract mushroom spores? ›

Put a drop of water on the top of the cap to help release the spores. Cover the cap with a paper cup or glass and leave for 2-24 hours, depending on the humidity and the freshness of the mushroom. The spores will fall on the paper, foil or glass, making a spore print pattern.

What should I do with my spore syringe? ›

Heat the needle of your spore syringe in a flame and let it cool. Inject around 4ml of spore solution into each jar. You should distribute this over multiple holes, and try to get it down the inside of the jar so it spreads better. If using micropore tape, replace it again once you remove the needle.

Can you inoculate grain with spore syringe? ›

You can also inoculate sterilized grain from a syringe- either a liquid culture or a spore syringe. In order to do this, you first need to flame sterilize the tip of the syringe until it is red hot. Then you can quickly lift the lid of the grain jar and inject the syringe.

What is the difference between liquid culture and spore syringe? ›

Liquid Culture vs.

spores revolves around speed and diversity. Liquid culture accelerates mycelium growth, boasts higher success rates, and lowers contamination risks—ideal for beginners and seasoned growers. Spores, however, embrace genetic diversity, paving the way for a more organic cultivation process.

How long does liquid culture last in a syringe? ›

Use within a reasonable timeframe: Liquid culture can be stored for several months, but it is best to use it within a few weeks to ensure the best results.

How can I speed up mycelium growth? ›

The higher the inoculation rate or amount of spawn added to the substrate, the faster the mycelium will grow through the substrate. The substrate likely will not produce a larger amount of mushrooms, so a high inoculation rate means more money spent on spawn per pound of mushrooms.

What do I need to make a spore syringe? ›

To make a spore syringe, you are going to need distilled water, a glass, used or brand-new syringes, metal utensils, and a pressure cooker. First, fill the shot glass or regular-sized glass with distilled water, using a little bit more water than what you will need to fill up all your spore syringes.

How do you make mushroom spore plugs? ›

Mushroom Plugs – Step By Step
  1. Step 1: Soak Plugs for 24 hours. Soak the dowels in clean water for about 24 hours. ...
  2. Step 2: Drain and Sterilize. After a 24 hour soak, the dowels should be sufficiently hydrated. ...
  3. Step 3: Inoculate Plugs. ...
  4. Step 4: Colonize, Then Use or Store.

What is in mushroom spore syringe? ›

Mushroom spores suspended in sterile water inside of a syringe are called spore syringes. Both can be used to cultivate mushrooms by injecting a portion of the syringe into a sterilized grain bag which colonizes and can then be transferred to sterile substrates such as manure, straw, or sawdust.

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